Career objective :
To seek a position of Bio-tech specialist in one of the Bio-tech firm where I can use my academic qualification for the benefit of the company. I am quite confident that my creative ideas as well as theoretical knowledge will help me to take the company to great heightsArea of Interest : Quality control, Quality assurance, Research and Development.Academic Qualifications :
Degree Board/University %CGPA Class
M.Sc.Biotechnology KIIT University,Bhubaneswar 7.84 First
B.Sc.Biotechnology Sikkim Manipal University,Central IT college 74.75 First
10+2 AHSEC,Assam 53.0 Second High School SEBA,Assam 70.83 FirstResearch/Project experience/s :
A miniproject had been done in Microbiology subject on Isolation, characterization, identification and enumeration of bacteria from waste water sample under Dr. Priyadarshi Sahu at KIIT University.
A 6-month project had been done on CEES enhances the cytotoxicity in macrophages cell under Dr. Arttatrna Pal at KIIT School of Biotechnology.Description :
CEES [2-chloroethyl ethyl sulfide] , a chemical warfare bifunctional alkylating agent,which plays an important role in SM-linked oxidative stress.SM exposure can cause significant blistering of the skin, respiratory injury, and fibrosis. CEES produces a large amount of ROS, makes the cellular defence mechanism weak and plays a major role in the development of lung injury and different forms of cancer.Due to this oxidative stress,there is an imbalance between the generation of oxidants and the oxidant scavenging molecules.
The main aim was to study the effect of SM(i.e to see the cytotoxicity in macrophages cell) and evaluate the oxidative stress. We also focused on the activation of few pathways which were triggered due to antioxidant condition because an antioxidant neutralizes the free radicals. Finally COMET and DAPI staining technique was performed that due to high dose of CEES treatment, under normal condition ,the DNA of the cells are intact,but when we increases t Sponsored links :
he dose of CEES, it shows that the DNA gets fragmented.It indicates that high dose of CEES, imparts DNA damage directly or indirectly,which results in the death of the macrophages cell by apoptosis.Skills developed during project :
MTT assay for cell viability
Nuclei staining by DAPI method
COMET assay for DNA fragmentationLaboratory Skills :
Biochemistry and Immunological techniques- Antigen and Antibody Interaction, ELISA
Microbial techniques- Spreading, Pouring, Streaking, Gram’s Staining, Biochemical reactions etc.
Molecular Biology and recombinant DNA technology- DNA isolation, SDS-PAGE, Restriction Digestion, PCR (including colony PCR sequencing PCR), Cloning, Transformation, Agarose Gel Electrophoresis, western blotting etc.
Bioinformatics- Softwares such as EMBOSS, RasMol, ExPASy, Chimera,TMHMM,KEGG,BLAST,PHYLO,HYPERCHEM,HOMO LOGY MODELING, MOLEGRO VIRTUAL DOCKER etc.
Plant tissue culture- Anther culture, embryo culture, callus culture.Special skills :Computer/Technical skills :
PerlLanguage proficiency/Communication skills :
Good communication skill in English, Hindi, Assamese.
Good presentation skill(power point presentation and documentation)Workshops and Conferences :
Participated in the 37th annual conference of Environmental Mutagen Society of India (EMSI) 2012 held in KIIT university, Bhubaneswar.
Indian immunology society Odisha chapter.
Attended workshop on “Management of University Patenting”.
Participated in 53rd annual conference of Association of Microbiologist of India (AMI) 2012 held in KIIT University, Bhubaneshwar.Extracurricular activities/interests :Hobbies : Enhancing my skill in my free time, gardening.Personal Details :Nationality : IndianDeclaration :
I hereby declare that the information furnished above is true to the best of my knowledge and belief.Place : BhubaneswarDate :
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