Gazi MD. Noor Uddin :
DVM, MS, PhD Candidate, Copenhagen University, Denmark
Food Safety and Zoonoses
Department of Veterinary Disease Biology
Faculty of Health and Medical Sciences
University of Copenhagen, Denmark
Mobile : +4571618258
Email : nug AT sund.ku.dk
gmnuvetmicro AT gmail.com
EDUCATION :
PhD (2015) : Microbiology (PhD thesis-accepted for public defense on 21.04.2015), Copenhagen University, Denmark
Major Research a PhD : Bacterial antimicrobial resistance and food safety in cultured shrimp
Master’s degree (2006) : Microbiology, Bangladesh Agricultural University, Bangladesh
Major Research at Masters Level : Virology (Avian Influenza virus)
Veterinary degree (2004) : DVM, Chittagong Govt. Veterinary College (Recently Chittagong Veterinary and Animal Sciences University), University of Chittagong, Bangladesh
AMBITIONS :
a. Determined to build research-based academic carrier
b. Research will be focused on antimicrobial resistance and public health
c. In order to conduct wide ranges of reasearch on antimicrobial resistance and public health, I am ambitious to form a vibrant research group
SCIENTIFIC SKILLS :
A) Well-experienced in characterizing a range of bacteria, including pathogens, by a variety of phenotypic and modern molecular techniques, including within the area of antimicrobial resistance.
The bacteria are mentioned below :
a. Bacterial analysis of wide range of food and probiotic products, e.g. seafood, juice, milk and analysis of antimicrobial resistance in food products :
1. Nosocomial pathogens, e.g. Acinetobacter spp., Staphylococcus spp.
2. Zoonotic pathogens such as Salmonella spp., Shigella spp.
3. Other human pathogens (Gram-negative and Gram-positive), e.g. Klebsiella spp. and Aerococcus urinaeequi
4. Wide range of normal bacterial flora of seafood (24 genus and 51 species)
5. Wide range of Bacillus spp. (15 Bacillus species) in probiotic products
B) Well-experienced with the following latest scientific methods :
a. Next generation sequence analysis
b. 16S rRNA gene sequence analysis
c. gyrB gene sequence analysis
d. Multi-plex PCR
e. Conventional PCR
f. Pulse-Field Gel Electrophoresis (PFGE)
g. MALDI-TOF Method
h. Conventional method od bacterial culture, isolation and identification
i. Application of common bioinformatics tools in microbial genomics
j. DNA extraction procedures
k. qPCR method
C) Experienced in bioinformatics activities includoing DNA sequencing, database, pairwise alignment and BLAST, multiple alignment, phylogeny, primer design, genomics and molecular identification of bacteria.
D) Experienced with the following bioinformatics tools :
1. Emboss program (water and needle)
2. ORF finder from NCBI
3. CLC genomic workbench
4. megaBLAST
5. MEGA5
6. RAST
7. ResFinder
LIST OF PUBLICATIONS :
List of publications, theses and project report are given in the Appendix-2
CONFERENCE AND SYMPOSIUM ATTENDED :
1. Conference of World Aquaculture Society : 1 September to 7 September 2012. Presented the results of one of my study entitled “Bacterial diversity and antimicrobial resistance in raw frozen cultured seafood imported to Denmark”.
2. Copenhagen Microbiology Centre (CMC) Symposium : Attended CMC symposium at Copenhagen on 6 October 2012. Poster presented.
3. Aquaculture and Environment : A focus in the Mekong Delta, Vietnam, April 3-5. One of my PhD study result entitled ‘Prevalence and antimicrobial resistance of Salmonella spp. in extensive and intensive cultured shrimp in the Mekong Delta, Vietnam has been presented in “Aquaculture and Environment” conference in Vietnam (Conference held in Can Tho University from 4-5, April/2014).
RESEARCH AND WORK EXPERIENCES :
I am equipped with 10 years research experiences in clinical and food microbiology in the Asian (Bangladesh, Vietnam) Universities, dairy industries (Bangladesh) and Danish University. I have published 15 peer-review articles (including upcoming articles) in the reputed journals where I contributed as first author in 7 articles, senior author in 2 articles and second/third co-author in 6 articles and attended conferences for dissemination of research findings across the world. I have also managerial experience for four years where I served as QA manger for 1.5 year and Factory manager for 2.5 years in Dairy industries. Details on research and work experience have been given in the Appendix-1.
CURRENT RESEARCH :
I am dealing with food safety issues in particular antimicrobial resistance in seafood. There are limited information on antimicrobial resistance in seafood, lack of data quantittative microbial risk assessment (QMRA) data and lack of indicator bacteria for monitoring antimicrobial resistance in seafood compared to E. coli and entrococci in other meat products, e.g. beef, pork and poultry. These issues are clearly associated with public health. My current research intended to determine a single bacterial indicator of antimicrobial resistance in seafood, bacterial antimicrobial resistance and food safety in seafood.
Intensive aquaculture use probiotics for disease prevention and control. It is not known well about the role of probiotic bacterial strain in disseminating antimicrobial resistance in cultured organism and in the aquaculture environment. In this context, I have conducted another study that deals with antimicrobial resistance in Bacillus probiotic bacterial strains used in shrimp culture. Whole genome sequencing results revealed that they contained few number and types of antimicrobial resistance genes.
Salmonella spp. is often reported to be identified from seafood all over the world. Occurrence of Salmonella spp. in seafood is mainly associated with contamination from faecal sources either in the pond or during harvesting, processing. Occurrence of Salmonella spp. in seafood is associated with food safety issues. During my PhD study, I found Salmonella spp. occurred in harvested shrimp from a single clone.
GNERAL INTEREST INCLUDE TO ENSURE MICROBIAL FOOD SAFETY :
(1) Antimicrobial resistance in food chain as well as human and animal pathogens; (2) Antimicrobial resistance in probiotics; (3) Probiotic biology particularly Bacillus and Lactobacillus biology; (4) Bacterial diversity and genomics, (5) Biocide resistance, (6) Survivability of Salmonella spp. in the pond environment, (7) Biofilm research, (8) Preventive Medicine (Vaccinology).
TEACHING ACTIVITIES :
Teaching activities are mainly related with knowledge dissemination through oral presentation, poster presentation in different conferences and symposiums. I had to train the new PhD students of our research group about the laboratory techniques and other issues or techniques related to the experiments.
Moreover I had to offer traing course on Hygienic milk production during working in the dairy industry to the Quality Assurance personnels, Factory people and the farmers.
FUTURE TEACHING PLAN :
I will offer research based teaching to the undergraduate and post-graduate students.
I will offer the following courses to the undergraduate and post-graduate students :
a. Clinical Microbiology : This course will offer knowledge on biology of clinically relevant bacteria including zoonotic pathogens, e.g. Salmonella spp. to the undergraduate and post-graduate students.
b. Infection Microbiology : This course will be offered to the post-graduate students. This course will enable students to learn wide range of molecular methods to type, identify and epidemiology of wide range of bacteria. Bacterial cloning and gene expression will also be included in this course.
c. Diagnostic Microbiology : This course will be offered to the undergraduate and post-graduate students. This course will be focused on sample or specimen collection, preservation and preparation for identification of wide range of bacteria of clinical importance. Students will be taught with bacterial characterization and identification using latest molecular methods, e.g. Next generation sequencing.
d. One Health-Food chain : This course will mainly focus on transmission of antimicrobial resistant zoonotic pathogens, e.g. Salmonella spp., Campylobacter spp. and E. coli and other antimicrobial resistant bacteria from animal originated food products (e.g. seafood, pork, beef, poultry, milk, eggs) or other food products to human. This course will also provide knowledge on antimicrobial resistance monitoring in various chain of bacterial transmission, e.g. food chain. This course will be offered to the undergraduate medical students. e. Application of Bioinformatics in Microbiology: This course will be offered to the under-graduate and post- graduate students. This course will enable the students to type diversified bacteria using DNA sequences and analyze bacterial diversity. The students will gain knowledge on designing of primer, preparation of phylogenetic tree and overall, they will be able to handle and manage bacterial DNA sequences using a variety of bioinformatics tools.
Outcome of these courses :
Knowledge offered through the above mentioned courses will enable future doctors, researchers and academic physicians to develop skill in diagnosis of infections.
In addition, they will learn about antimicrobial resistant bacteria with their impact on human health and method of treatment, i.e. drug selection and rational use of antimicrobials in treating infections. Knowledge of "One Health-Food chain" course will help them to understand the dynamics of transmission of antimicrobial resistant zoonotic pathogens, e.g. Salmonella spp. and other antimicrobial resistant bacteria and they will be able to play a role in controlling human infections with antimicrobial resistant bacteria. Furthermore, they will be able to take part in research on antimicrobial resistance monitoring in food chain and in the human health care settings, such as antimicrobial resistance in nosocomial pathogens including Acinetobacter spp. and Staphylococcus species.
FUTURE RESEARCH PLAN :
Overall, I am determined to continue my future research on zoonoses, food safety, bacterial genomics, antimicrobial resistance, biocide resisstance and biofilm formation and other allied filed of biomedical sciences. In addition, I am enthusiastic to conduct basic research in future.
MY COLLABORATIONS :
a. Department of Veterinary Disease Biology, Faculty of health and Medical Sciences, University of Copenhagen
b. Department of Biomedical Sciences and Microbiology, Oregon State University, USA
c. College of Aquauclture and Fisheries, Can Tho University, Vietnam
d. Department of Food Microbiology, Helsinki University, Finland
e. Different Universities in Bangladesh
f. International Centre for Diarrheal Disease Research, Bangladesh (www.icddrb.org)
REVIEW ACTIVITIES :
I am reviewer of International Journal of Dairy Science and American Journal of Food Microbiology
REFEREES :
1. Anders Dalsgaard, PhD :
Professor
Food Safety & Zoonoses section
Department of Veterinary Disease Biology
Faculty of Health and Medical Sciences
Copenhagen University, Denmark
E-mail : adal AT sund.ku.dk
2. Mahfuzur Sarker, PhD :
Professor
Departments of Biomedical Sciences and Microbiology
Oregon State University
216 Dryden Hall
Corvallis, OR97331, USA
E-mail : sarkerm AT oregonstate.edu
3. Henrik Christensen, PhD :
Associate Professor
Veterinary Clinical Microbiology section
Department of Veterinary Disease Biology
Faculty of Health and Medical Sciences
Copenhagen University, Denmark
E-mail : hech AT sund.ku.dk
Appendix-1 :
PERSONAL INFORMATION :
NATIONALITY : Bangladeshi (by birth)
RESIDENT : Danish Green Card holder
LANGUAGE : Bengali (Mother Language), English (2nd Language), Danish (Level 2)
RESEARCH AND WORK EXPERIENCES :
RESEARCH EXPERIENCE IN THE UNIVERSITIES OF BANGLADESH :
SCIENTIFIC OFFICER : I served as Scientific Officer in the Department of Microbiology of Chittagong Veterinary and Animal Sciences University (CVASU) (former CGVC) from 1/03/2003 to 30/11/2003. My responsibility was to diagnose diseases causing mortality of poultry.
RESEARCH FELLOW : I served as Research Fellow in Smallholder Livestock Development Project-2 (SLDP-2) under the Dept. of Microbiology & Hygiene of Bangladesh Agricultural University (BAU), Mymensingh from 1/07/2005 to 30/06/2006 to conduct research for achieving Master of Science in Microbiology.
SCIENTIFIC OFFICER : I served as Scientific Officer in the Dept. of Dairy & Poultry Science of CVASU from 1/04/2006 to 5/09/2006.
DAIRY INDUSTRIAL EXPERIENCES IN BANGLADESH :
HEAD OF QA DEPARTMENT : I served as head of Quality Assurance department of PRAN Dairy Ltd (Bangladesh) for 1.5 year (from 06/09/2006 to 04/03/2008). The major responsibilities included ensure bacteriological and chemical quality of raw milk, pasteurized milk and UHT milk, yoghurt and mango juice.
HEAD OF DAIRY FACTORY : I served as Factory Manager of AFTAB MILK & MILK PRODUCTS LTD. for 2.5 years (from 05/03/2008 to 30/09/2010). The main responsibilities included quality assurance and production operation of pasteurized, UHT milk and other milk products.
RESEARCH EXPERIENCE IN COPENHAGEN AND CAN THO UNIVERSITY :
I served as PhD student and Research Assistant (employed position) in the Dept. of Veterinary Disease Biology, Faculty of Health and Medical Sciences, Copenhagen University, Denmark since 15/10/2010. As a part of my PhD research, I had opportunity to conduct research work in Can Tho University in Vietnam for one moth (10/10/2013-10/11/2013). During my PhD, I also attended Helsinki University two times to participate in course work. In addition, I have attended Chittagong Veterinary & Animal Sciences University, Bangladesh Agricultural University, Tamil Nadu Veterinary & Animal Sciences University for Bachelor and Master’s education and training.
Appendix-2 :
Articles to be generated from PhD study (3 articles) :
1. G.M.N. Uddin, M.H. Larsen, T.M Phu and A. Dalsgaard (2014) : Clonal occurrence of Salmonella spp. in cultured shrimp in the Mekong Delta, Vietnam (Submitted to PLoS ONE). (5 years impact Factor: 4.24).
2. G.M.N. Uddin, M.H. Larsen, H. Christensen, T.M Phu and A. Dalsgaard (2014) : Antimicrobial resistance in bacteria isolated from probiotics used in shrimp culture (Accepted with minor revision in PLoS ONE). (5 years impact Factor: 4.24).
3. G.M.N. Uddin, M.H. Larsen, L. Guardabassi and A. Dalsgaard (2013) : Bacterial flora and antimicrobial resistance in raw frozen cultured seafood imported to Denmark. Journal of Food Protection, 76 (3): 490-499. (5 year Impact Factor: 2.175)
Others :
4. T. Bilkis, M.M. Islam, M.C. Sumy, M.N.A. Mandal and G.M.N. Uddin (2013) : Rapid estimation of quality of raw milk for its suitability for further processing in the Dairy industries of Bangladesh. International Journal of Dairy Science, 8 (1):1-11.
5. T. Bilkis, M.K.I. Khan, M.S. Rahman and G.M.N. Uddin (2013) : Inheritance pattern of sex-linked trait and feeding and sexual behaviour of Drosophila melanogaster. International Journal of Pure and Applied Zoology, 1(4): 337-343.
2009 :
1. P.K. Biswas, H. Barua, G.M.N. Uddin, D. Biswas, A. Ahad and N.C. Debnath (2009) : Serosurvey of five viruses in chickens on smallholdings in Bangladesh. Preventive Veterinary Medicine, 88 (2009) 67-71. (5 year Impact Factor: 2.565)
2008 :
1. P.K. Biswas, G.M.N. Uddin, H. Barua, K. Roy, D. Biswas, A. Ahad and N.C. Debnath (2008) : Survivability and causes of loss of broody-hen Chicks on Smallholder households in Bangladesh. Preventive Veterinary Medicine, 83 (2008) 260-271. (5 year Impact Factor: 2.565)
2. G.C. Chanda, G.M.N. Uddin, A. Deb, S. Chowdhury and T. Bilkis (2008) : Microbiological Profile of the traditionally collected industrial raw milk from the milk pocket zones of Bangladesh. Bangladesh Journal of Microbiology, 25 (1): 17-20.
2007 :
1. G.M.N. Uddin, M. T. Rahman, M. Mohammad and M. M. Rahman (2007) : Identification and Characterization of Hemagglutinating Viruses in Native Chickens in Bangladesh. International Journal of Poultry Science, 6(12): 912-915, 2007.
2. G.C. Chanda, G.M.N. Uddin, A. Deb, S. Chowdhury and T. Bilkis (2007) : Physical and Chemical quality of market milk (Cow’s milk) produced by the private dairy farms in the Chittagong Metropolitan area, Bangladesh. Journal of Agricultural Education and Technology, 10 (1&2): 127-130, December 2007.
2006:
1. G.M.N. Uddin and Biswas, P.K. (2006): Identification of Leptospires in the Urine samples of Cattle and Dogs registered at Veterinary Hospital using modified silver staining technique of Fontana. Bangladesh Journal of Microbiology, 23(1): 1-3.
2. G.M.N. Uddin, M. T. Hossain, M. Mohammad and M. M. Rahman (2006): Sero-Epidemiology of Avian Influenza Virus in Native Chicken in Bangladesh. International Journal of Poultry Science, 5(11): 1029-1033.
3. P.K. Biswas, G.M.N. Uddin, H. Barua, K. Roy, D. Biswas, A. Ahad and N.C. Debnath (2006): Immune status of semi-scavenging Sonali chickens in Bangladesh against Newcastle disease. Journal of Livestock Research for Rural Development, 18 (6), June 2006.
4. P.K. Biswas, G.M.N. Uddin, H. Barua, K. Roy, D. Biswas, A. Ahad and N.C. Debnath (2006): Causes of loss of Sonali chickens on smallholder households in Bangladesh. Preventive Veterinary Medicine, 76 (2006) 185-195. (5 year Impact Factor: 2.565)
5. G.M.N. Uddin, G.C. Chanda, A. Deb, S. Chowdhury and T. Bilkis (2006): Microbiological and Hygienic quality of the market milk (Cow’s milk) produced by the private dairy farms in Chittagong Metropolitan area of Bangladesh. Journal of Agricultural Education and Technology, 9 (1&2): 131-136, December 2006.
RESEARCH REPORT AND THESIS:
1. “Bacterial antimicrobial resistance and foof safety in cultured shrimp”. (PhD Thesis-accepted for public defense)
2. “Seroepidemiology of Avian Influenza and detection of its agent in native chickens of SLDP-2 area”. (Master’s Thesis)
3. Final report on “A Longitudinal Study to identify the Causes on the Mortality of Sonali Birds and Broody Hen Chicks of Key Beneficiaries in the SLDP-2 Area.” DANIDA, 2004. (Project Report)