Objective :-
To apply my knowledge and experience in solving biological and developing sustainable and novel technology.
Education :-
** Bachelor of science in Biotechnology (on going 6 sem) (july 2016 to april 2019)
** Rajiv gandhi institute of information technology and biotechnology,Pune.
** Intermediate education (BSCB) (april 2014 to may2016)
** Jaglal roy college ,chhapra ,Bihar.
** High school (CBSE) (May 2013)
** R.P.S Residential public school , Patna ,Bihar .
Relevant Skill :
Chemistry :
** Basic operation and maintenance of spectrophotometer (i.e Spec 21 and UV- vis) to monitor the growth of microbial culture and to assay enzymatic activities.
** Skillful in preparation of various stock solution and reagents including anaerobic stock.
Microbiology :
** Preparation of various media including anaerobic to support the growth of microorganism and operation of autoclaves.
** Sterile technique in isolation add transfer of microorganisms.
** Maintenance and storage of aerobic and anaerobic microbial culture.
** Basic operation and maintenance of bright field, fluorescent and phase contrast microscope.
** Basic staining technique and performing various microbiological tests like IMVAC.
** Preparation of various fungal media and handling of pathogenic strains of fungi.
Molecular Biology :
** Gel Electrophoresis of DNA and RNA.
** SDC PAGE electrophoresis techniques.
** PCR including inverse PCR, Real-Time PCR, Reverse Transcriptase PCR, Nested PCR.
** RAPD and RELP.
** Site-Directed and Random Mutagenesis using Chemical and PCR methods.
** Pulse Field (CHEF) Gel Electrophoresis.
** Northen, Western and Southern Blots using radioactive and non-radioactive Probes.
** DNA and RNA extraction from eukaryotic and prokaryotic cells including the whole chromosome extraction.
** Achilles Heel RecA mediated cleavage of whole chromosomes.
** Restriction Digestion, Ligation, Cloning, and Transformation techniques.
** Two hybrid colony screening
** Proficient in use of recombineering technologies like Cre-Lox, GATEWAY, TOPO cloning.
** Co-precipitation of proteins.
** Expression and isolation of Tagged Protein from Bacterial and Eukaryotic cells.
** Sequencing using automated sequencers and interpretation and assembly of contigs.
** Handling of phage and eukaryotic vector.
** Proficient in FISH techniques and various other assays.
** Plant tissue culture.
** Extensive knowledge in various Bioinformatics tools and services like NCBI, Swiss PDB ,EMBL ,RasMol,BLAST,CLUSTALW,VectorNti.
Computer :
** Proficiency in operation of personal computer and software package like microsoft office ,
** Use of MS excel to create spreadsheet,graph and statistically analyze data (i.e.
average,standard deviation ,student t-test ,linear regression analysis).
** Literature searches throgh program such as PubMed.
** Use bibliography management tools like Endnote.
Supervisory skills :
** Managed group lab sessions and evaluated a total of 180 undergraduate student over 5 semester.
** Monitored activity of student and ensured safety provisions were used.
** Ordered lab supplies and maintained general business of the lab.
Conference and workshop :-
1.Co-founder, sustainable living integrated solution, member of arthakrani movement, member of ecological society.(Mr.Bhushan Patil) [17th Feb 2018].
2.An insider view on DNA polymerase Dr.Mukund Modak , Rutgers university , New Jersy, USA [7th Jan 2019]
3."Academic Research , development and Innovation " Dr.Mahesh Deshpande founder and director ,research development and Innovation Lab LLP (RDIL),PUNE [10th jan 2019]
4.Concept of Commercialization by Emeritus scintist , Dr. Mukund Deshpande national cgemical laboratory(NCL), [9th feb 2019].
Date :
Place :